Application of in vitro culture techniques in the propagation of alder for their conservation

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Published: 30-04-2012

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Elena Corredoira
Departamento de Fisiología Vegetal, Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda de Vigo s/n., Apartado 122, 15080 Santiago de Compostela
Spain
Laura V. Janeiro
INLUDES. Diputación Provincial de Lugo, Ronda da Muralla 140, 27004 Lugo
Spain
María C. San José
Departamento de Fisiología Vegetal, Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda de Vigo s/n., Apartado 122, 15080 Santiago de Compostela
Spain
No 7 (2011), Original articles
DOI: https://doi.org/10.15304/rr.id92
Submitted: 30-04-2012 Accepted: 30-04-2012 Published: 30-04-2012
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Abstract

An efficient protocol was developed for the invitro establishment of axillary buds from material obtainedfrom mature alder specimens of different genotypes. Thecuttings, obtained from 20 to 30 year-old-trees, were forcedflushed and the shoots were cultured in Woody PlantMedium (WPM) (Lloyd and McCown, 1980) with 2 mg/lbenzyladenine (BA) and 0.5 mg/l indol-3-acetic acid (IAA).The cultures were maintained by sequential subcultureevery 9 weeks on the same mineral medium, but with 0.1mg/l BA and 0.5 mg/l IAA. Transfer to fresh medium every 3weeks, the inclusion of zeatin (Z) in the final cycle andsubstitution of 3% sucrose with 2% glucose improved therates of multiplication and the quality of the shoots. Theshoots obtained at the multiplication stage rooted easily,even without auxin (84.0%), although the presence of indol-3-butiric acid (IBA) in the medium increased the rootingsuccess, especially when glucose 2% was used (93.4%) inplace of sucrose 3% (86.9%). The rooted plants wereacclimatized in a growth chamber for 6 weeks before beingtransferred to the greenhouse.

Keywords:

Alnus glutinosa, black alder, germplasm conservation, micropropagation, Phytophthora alni
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