Extracción de polifenoles bioactivos del bagazo de uva mediante un método de dispersión de matriz en fase sólida

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Marta Lores
Universidad de Santiago de Compostela. Facultad de Química. Departamento de Química Analítica, Nutrición y Bromatología
España
María Iglesias Estévez
Universidad de Santiago de Compostela. Facultad de Química. Departamento de Química Analítica, Nutrición y Bromatología
España
Marta Álvarez Casas
Universidad de Santiago de Compostela. Facultad de Química. Departamento de Química Analítica, Nutrición y Bromatología
España
María Llompart
Universidad de Santiago de Compostela. Facultad de Química. Departamento de Química Analítica, Nutrición y Bromatología
España
Carmen García Jares
Universidad de Santiago de Compostela. Facultad de Química. Departamento de Química Analítica, Nutrición y Bromatología
España
No 8 (2012), Artigos orixinais
DOI https://doi.org/10.15304/rr.id790
Recibido: 22-01-2013 Aceptado: 22-01-2013
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Resumo

A matrix solid-phase dispersion process to extract polyphenols from grape marc, a winemaking byproduct, has been optimized by Response Surface Methodology. The dependent variables evaluated were the total polyphenols content, flavanols, hydrocynnamates, and flavonoids. The performance of the extraction method in terms of linearity and precision has also been assessed. The optimized MSPD method provides good results to extract polyphenols from white winemaking byproducts, much less studied than those from red wines, requiring low solvent consumption and being low cost and fast (15 min). The analytical instrumentation is available almost everywhere, thus becoming a ready-to-use methodology for virtually any control or winery laboratory. The optimized method has been applied to a set of bagasse samples from Albariño grapes (Vitis vinifera sp) cultivated in Galicia (NW Spain) and coming from five different types of grapevine training techniques. The vine training factor was significant on the basis of the content of the different groups of polyphenols determined in the MSPD extracts.

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Grape pomace, Matrix solid-phase dispersion, Polyphenols, Vine training techniques, Winemaking byproducts
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Albero, B., Sánchez-Brunete, C., Donoso, A., Tadeo, J. L. (2004). Determination of herbicide residues in juice by matrix solid-phase dispersion and gas chromatographymass spectrometry. Journal of Chromatography A. 1043, 2: 127-133.

Alonso, A. M., Guillén, D. A., Barroso, C. G., Puertas, B., García, A. (2002). Determination of Antioxidant Activity of Wine Byproducts and Its Correlation with Polyphenolic Content. Journal of Agricultural and Food Chemistry. 50, 21: 5832-5836.

Barker, S. A. (2000). Matrix solid-phase dispersion. Journal of Chromatography A. 885, 1-2: 115-127.

Barker, S. A. (2007). Matrix solid phase dispersion (MSPD). Journal of Biochemical and Biophysical Methods. 70, 2: 151162.

Barker, S. A., Long, A. R., Short, C. R. (1989). Isolation of drug residues from tissues by solid phase dispersion. Journal of Chromatography A. 475, 2: 353-361.

Baydar, N. G., Özkan, G., Sagdiç, O. (2004). Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control. 15, 5: 335-339.

Bogialli, S., Di Corcia, A. (2007). Matrix solid-phase dispersion as a valuable tool for extracting contaminants from foodstuffs. Journal of Biochemical and Biophysical Methods. 70, 2: 163-179.

Dopico-García, M. S., Valentão, P., Jagodziñska, A., Klepczyñska, J., Guerra, L., Andrade, P. B., Seabra, R. M. (2007). Solid-phase extraction versus matrix solid-phase dispersion: Application to white grapes. Talanta. 74, 1: 2031.

El Gharras, H. (2009). Polyphenols: food sources, properties and applications – a review. International Journal of Food Science & Technology. 44, 12: 2512-2518.

FAO. (2011). FAO STAT http://faostat.fao.org. access 30/2011.

Fernández, M., Picó, Y., Mañes, J. (2000). Determination of carbamate residues in fruits and vegetables by matrix solidphase dispersion and liquid chromatography-mass spectrometry. Journal of Chromatography A. 871, 1-2: 4356.

Gonzalez, C., M, R., Rossello, C, Simal, S, Garau, M, C., Lopez, F, Femenia, A. 2010. Physico-chemical properties of cell wall materials obtained from ten grape varieties and their byproducts: grape pomaces and stems. Elsevier, Kidlington, ROYAUME-UNI.

Ignat, I., Volf, I., Popa, V. I. (2010). A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry. 126, 4: 1821-1835.

Kammerer, D., Claus, A., Carle, R., Schieber, A. (2004). Polyphenol screening of pomace from red and white grape varieties (Vitis vinifera l.) by HPLC-DAD-MS/MS. Journal of Agricultural and Food Chemistry. 52, 14: 4360-4367.

Kim, D.-O., Chun, O. K., Kim, Y. J., Moon, H.-Y., Lee, C. Y. (2003). Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry. 51, 22: 6509-6515.

Kristenson, E. M., Brinkman, U. A. T., Ramos, L. (2006). Recent advances in matrix solid-phase dispersion. TrAC Trends in Analytical Chemistry. 25, 2: 96-111.

Lagunas-Allue, L., Sanz-Asensio, J., Martinez-Soria, M. T. (2010). Response surface optimization for determination of pesticide residues in grapes using MSPD and GC-MS: assessment of global uncertainty. Analytical and Bioanalytical Chemistry, 398, 3: 1509-1523.

Lee, J., Rennaker, C. (2011). Influence of extraction methodology on grape composition values. Food Chemistry. 126, 1: 295-300.

Lian, Y.-J., Pang, G.-F., Shu, H.-R., Fan, C.-L., Liu, Y.-M., Feng, J., Wu, Y.-P., Chang, Q.-Y. (2010). Simultaneous determination of 346 multiresidue pesticides in grapes by PSA-MSPD and GC-MS-SIM. Journal of Agricultural and Food Chemistry. 58, 17: 9428-9453.

Liu, E. H., Qi, L.-W., Cao, J., Li, P., Li, C.-Y., Peng, Y.-B. (2008). Advances of modern chromatographic and electrophoretic methods in separation and analysis of flavonoids. Molecules. 13, 10: 2521-2544.

Makris, D. P., Boskou, G., Andrikopoulos, N. K. (2007a). Polyphenolic content and in vitro antioxidant characteristics of wine industry and other agri-food solid waste extracts. Journal of Food Composition and Analysis. 20, 2: 125-132.

Makris, D. P., Boskou, G., Andrikopoulos, N. K. (2007b). Recovery of antioxidant phenolics from white vinification solid by-products employing water/ethanol mixtures. Bioresource Technology. 98, 15: 2963-2967.

Manhita, A. C., Teixeira, D. M., da Costa, C. T. (2006). Application of sample disruption methods in the extraction of anthocyanins from solid or semi-solid vegetable samples. Journal of Chromatography A. 1129, 1: 14-20.

Minuti, L., Pellegrino, R. (2008). Determination of phenolic compounds in wines by novel matrix solid-phase dispersion extraction and gas chromatography/mass spectrometry. Journal of Chromatography A. 1185, 1: 23-30.

Montes, R., Canosa, P., Lamas, J. P., Pineiro, A., Orriols, I., Cela, R., Rodriguez, I. (2009). Matrix solid-phase dispersion and solid-phase microextraction applied to study the distribution of fenbutatin oxide in grapes and white wine. Analytical and Bioanalytical Chemistry, 395, 8: 2601-2610.

Psarra, E., Makris, D. P., Kallithraka, S., Kefalas, P. (2002). Evaluation of the antiradical and reducing properties of selected Greek white wines: correlation with polyphenolic composition. Journal of the Science of Food and Agriculture. 82, 9: 1014-1020.

Quideau, S., Deffieux, D., Douat-Casassus, C., Pouységu, L. (2011). Plant polyphenols: chemical properties, biological activities, and synthesis. Angewandte Chemie International Edition. 50, 3: 586-621.

Ramos, J. J., González, M. J., Ramos, L. (2009). Comparison of gas chromatography-based approaches after fast miniaturised sample preparation for the monitoring of selected pesticide classes in fruits. Journal of Chromatography A. 1216, 43: 7307-7313.

Sagdic, O., Ozturk, I., Ozkan, G., Yetim, H., Ekici, L., Yilmaz, M. T. (2011). RP-HPLC-DAD analysis of phenolic compounds in pomace extracts from five grape cultivars: Evaluation of their antioxidant, antiradical and antifungal activities in orange and apple juices. Food Chemistry. 126, 4: 1749-1758.

Singleton, V. L., Rossi, J. A., Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 3: 144-158.

Virot, M., Tomao, V., Le Bourvellec, C., Renard, C. M. C. G., Chemat, F. (2010). Towards the industrial production of antioxidants from food processing by-products with ultrasound-assisted extraction. Ultrasonics Sonochemistry. 17, 6: 1066-1074.

Xiao, H. B., Krucker, M., Albert, K., Liang, X. M. (2004). Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection. Journal of Chromatography A. 1032, 1-2: 117-124.

Yilmaz, Y., Toledo, R. T. (2003). Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid. Journal of Agricultural and Food Chemistry. 52, 2: 255-260.

Ziaková, A., Brandsteterová, E., Blahová, E. (2003). Matrix solid-phase dispersion for the liquid chromatographic determination of phenolic acids in Melissa officinalis. Journal of Chromatography A. 983, 1-2: 271-275.

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