Cladonia P. Browne is the genus with the highest number of species in the Cladoniaceae family . Identifying species within this genus is significantly difficult, as morphology varies notably during different stages of development and depending on environmental conditions. A wide variety of lichen substances have been identified in representatives of this genus, many can only be recognized through thin-layer chromatography (TLC) techniques. Although the identification of metabolites can be helpful, doubts and controversies regarding taxonomic status may still arise. Genetic tools, particularly in complex genera such as Cladonia, have significantly advanced the field. Molecular phylogenies were first attempted in the late 20th century, with STENROOS et al. , conducting the most extensive works on the genus to date. It is important to note that relying solely on the ITS region for taxonomic identification within this group may not always be effective. This is because species within the same clade may have very similar ITS regions, which can lead to misidentifications . Therefore, it should be complemented by morphological and chemical studies or additional genetic markers should be used.
conducted a comprehensive monographic study on the genus Cladonia in the Iberian Peninsula. This was followed by a study that included all species from the Mediterranean Region . The only previous monographic work on this genus in Galicia was carried out by in the municipality of A Fonsagrada, Lugo province. updated the collected material for the volume of the Iberian Lichen Flora, contributing 62 species of the genus Cladonia for Galicia and 46 for Pontevedra. According to , the most frequent and abundant species for Galicia are Cladonia furcata, Cladonia coniocraea, Cladonia rangiformis, Cladonia portentosa, and Cladonia pyxidata.
The study collected 300 specimens from various sampling points between September 2020 and March 2022, covering habitat variability. The voucher material has been deposited in the MACB herbarium of the Faculty of Biological Sciences at Complutense University of Madrid. The symbol ** is used to highlight new citations for the province of Pontevedra, * for new citations in the Galician community, and # for new chemotypes for the Iberian Peninsula.
Specimens of Cladonia were collected from various substrates in the municipality of O Rosal (Pontevedra) for a combined study of morphological, chemical, and molecular characters.
The study area is situated in the municipality of O Rosal, south of the province of Pontevedra, near the mouth of the Miño River. It covers an area of 41.13 km2 and consists of a small urban core, with mountainous areas located in two parallel strips along the coast up to 500 m above sea level. These strips delimit the valley through which the Tamuxe River flows. Geologically, the mountainous areas are composed of alkaline granites, which are also present in the valley alongside Holocene deposits. In addition, there are metavolcanic rocks and schists that create acidic substrates . Most of the mountainous areas are covered by plantations of Pinus pinaster, together with others that occupy a smaller area: Pinus radiata and Eucalyptus globulus. However, remnants of natural vegetation remain notably Quercus robur and Q. suber. The area is classified as a semi-hydro-oceanic bioclimate with a temperate thermoclimate according to the Rivas-Martínez climate classification. It is situated in a transition zone between the sub-Mediterranean temperate macroclimate and the typical temperate bioclimate, making it an area of special interest as it allows species from the two climates to coexist ; . The average annual rainfall is 1,573 mm/m2, and the average annual temperature is 15.0 ºC. The average temperature of the coldest month is 9.5 ºC, whereas the average temperature of the warmest month is 21.1 ºC.
All the sampled localities belong to the municipality of O Rosal, in the province of Pontevedra.
List of sampling localities:
Standard lichenology procedures were used to conduct morphological examination of specimens, using a Nikon SMZ445 stereomicroscope. Species identification primarily followed , with additional reference to other monographic works ; and general keys ; . Lichen substances were identified by thin-layer chromatography (TLC) techniques following the standardized method of , using solvents A, B, C and D; for substances not mentioned in this work and some doubtful cases, and were used.
For genetic analysis, several specimens of each identified species were selected based on their morphological and chemical traits, ensuring representation of those with divergences. The NZY PLANT/FUNGI gDNA ISOLATION kit (NZYTech, Portugal) was used for fungal gDNA extraction following the manufacturer's instructions. The resulting DNA was stored at -20 °C until PCR amplification. The ITS region of nrDNA was analyzed for species genetic characterization using the primers ITS1F and ITS4 . The amplification cycle was as follows: initial denaturation at 94 ºC for 5 min; 35 cycles of denaturation at 94 ºC for 30 s, annealing at 54 ºC for 30 s, and extension at 72 ºC for 40 s; and a final extension at 72 ºC for 7 min. Amplifications were visualized on 2% agarose gel with TBE 1X buffer and GreenSafe Premium (NZYTech, Portugal) for DNA visualization, run for 25 min at 100 V. Amplified DNA was sent to the Genomics Service of the Scientific-Technological Research Support Center (University of Vigo) for purification with ExoSap (ThermoFisher) and bidirectional sequencing using the BigDye Terminator v3.1 sequencing kit (Life Technologies) and a SeqStudio Genetic Analyzer capillary sequencer (Applied Biosystems). Sequencer files containing chromatograms were processed using the Geneious Global Alignment algorithm (Geneious, Biomatters) to generate consensus sequences. Initial identification was assigned to each sample using the Basic Local Alignment Search Tool (BLAST) nucleotide search to verify identity. BLASTn search was performed using the nucleotide collection (nr/nt). Sequences analyzed have been deposited in the GenBank genetic sequence database; the corresponding reference for each species is indicated in the database. Table 1 shows the sequences from this study alongside the species with the highest identity percentage after BLAST search, with any discrepancies noted for each species.
Of the 300 collected specimens, a total of 28 species of Cladonia were identified. The numbers of the locations correspond to the sampling localities list followed by the herbarium number where the specimens are deposited.
Despite significant human intervention, the study area displays a rich diversity of lichens . The surveyed area includes 28 species of the 62 Cladonia species cited for Galicia . Notably, the chemotype of Cladonia portentosa, containing fumarprotocetraric, perlatolic and usnic acids, is recorded for the first time in the Iberian Peninsula. Additionally, C. homosekikaica is a new addition to the Galician flora, and Cladonia ciliata is a new record for the Pontevedra province. The locality with the highest diversity is 'Petroglifos do Ghorghalado' (sample point 10), which is linked to areas with minimal human interference and a high level of moisture.
List of species.
Our specimens correspond to the chemotype: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. The specimens were found growing on soil among mosses. It is found in temperate and warm regions of the Northern Hemisphere . The species is widely distributed in the Eurosiberian Region, and has also been observed in other areas of the northern Mediterranean countries, such as southern France, northern Italy, Slovenia, and some isolated locations in northern Africa. In the Iberian Peninsula, it is mainly found in the northernmost areas ; .
Sampling collection: 10 (MACB 117654). GenBank accession number: OP479892.
(Fig. 1c The specimens are: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. Only two specimens were found on mossy rocks and soil. The species is not very abundant in the study area. It has a cosmopolitan distribution, although it is mainly concentrated in the Northern Hemisphere . In Europe, it is widely distributed, with a higher number of records in western and southern Mediterranean territories. In the Iberian Peninsula, it is also widely spread ; .
Sampling collections: 7 (MACB 117681, 117682). GenBank accession number: OP479903 for MACB 117681.
The species is easily confused with other closely related members, such as C. cryptochlorophaea Asah., C. merochlorophaea Asah., C. grayi G. Merr. ex Sandst., C. novochlorophaea (Sipman) Brodo & Ahti, and C. homosekikaica Nuno, due to many morphological similarities. Our specimens match the chemotype: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. Two specimens were found, on Alnus glutinosa and on Pinus radiata. This species has a subcosmopolitan distribution . It is widely distributed throughout the Mediterranean Region , with numerous records known from the Iberian Peninsula. However, its presence may be overestimated due to its similarity to other species mentioned above.
Sampling collections: 1 (MACB 117787), 13 (MACB 117788).
Our specimens are compatible with the f. flavicans. The specimens belong to the chemotype: Pd+ (red), K–, C–, KC–, UV–, containing usnic and fumarprotocetraric acids. Several specimens were found directly on mossy soil. The species is distributed throughout the Northern Hemisphere. In Europe, it can be found at practically all latitudes , f. flavicans is more abundant in the Mediterranean Region, where it occupies moister and warmer habitats than f. ciliata . In the Iberian Peninsula, most records are concentrated in the northern and western regions ; . This is the first record in Pontevedra province, although it has been cited in Lugo and A Coruña .
Sampling collections: 3 (MACB 117656), 10 (MACB 117657).
The specimens are: Pd–, K–, C–, KC+ (yellow), UV–, containing zeorin, usnic and rhodocladonic acids. Cladonia diversa, with very similar chemistry, is distinguished by its densely squamulose-covered podetia. Several specimens were found growing on mossy soil. It is a subcosmopolitan species , common up to the subalpine level of the Eurosiberian Region, while in the Mediterranean Region generally occurs at higher altitudes . In the Iberian Peninsula, most records are concentrated in the northern third .
Sampling collection: 4 (MACB 117655). GenBank accession number: OP479895.
(Fig. 1g) All the specimens are Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. In our case, ochrochlora morphology was observed on the ground and infrequently as an epiphyte, whereas specimens matching the coniocraea morphology were the most abundant, occurring on bark. of different trees (Quercus robur, Q. suber, Pinus pinaster and P. radiata) and occasionally directly on soil. The species has a wide distribution in the Northern Hemisphere, as reported by . In Europe, it is mainly found in the Eurosiberian Region, but it is also widely distributed in various locations of the Mediterranean Region . In the Iberian Peninsula, it has been reported several times, but most of the records are concentrated in the north ; .
Sampling collections: C. coniocraea s. str.: 1 (MACB 117661), 6 (MACB 117660), 7 (MACB 117662, 117663), 10 (MACB 117658, 117659), 15 (MACB 117664). C. coniocraea morphology C. ochrochlora: 10 (MACB 117747), 13 (MACB 117748), 15 (MACB 117749). GenBank accession number: OP479897 for MACB 117662.
(Fig. 1a. Our specimen is: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. A single specimen was found growing on soil. The occurrence of this species in the area is remarkable as it is common at higher altitudes . The molecular taxonomy techniques used to analyse the sequences resulted in a finding that is consistent with C. gracilis (L.) Willd., a species that has been found to be closely related in previous studies ; . However, the presence of soredia limited to the upper half of the podetia allows for its clear differentiation from the latter species. The taxon is subcosmopolitan, and more commonly found in cold regions of the Northern Hemisphere . It is also abundant in the Eurosiberian Region and present in some mountainous locations of the Mediterranean Region. In the Iberian Peninsula, the records are concentrated in the northwestern quadrant ; . It is reported from Ourense and reported this species in Pontevedra.
Sampling collection: 2 (MACB 117665).
Our specimens are: Pd+ (orange), K+ (ochre), C± (weak yellow, almost imperceptible), KC+ (wine red), UV+ (pale yellow), containing cryptochloropheaic and fumarprotocetraric acids. Several specimens grow on soil and trunks of Quercus robur, Pinus pinaster and P. radiata. It is the most common species in the study area. The species is mainly distributed in the Eurosiberian Region, but it can also be found in the territories of the Mediterranean Region. It has been reported in various locations in the northern and western areas of the Iberian Peninsula ; .
Sampling collections: 7 (MACB 117667), 10 (MACB 117666), 14 (MACB 117669, 117670, 117671, 117672), 15 (MACB 117668). GenBank accession number: OP479898 for MACB 117669.
(Fig. 1b). Our specimen is: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric and protocetraric acids. It is a rare species in this area. It grows on soil among mosses and on Pinus radiata, in the highest altitude of the municipality (527 m). C. cyathomorpha has a restricted distribution in Western Europe, reaching as far as the Canary Islands. It is probable that it has been disregarded or mistaken for other species within its group. Conversely, it has a broad distribution across the Iberian Peninsula .
Sampling collections: 1 (MACB 117789, 117790).
The specimen belongs to the chemotype: Pd+ (yellow), K+ (yellow), C–, KC–, UV–, containing thamnolic and rhodocladonic acids. Several specimens were found growing directly on soil, among mosses. The species is distributed across vast territories of the Northern Hemisphere . Its presence is considered a good indicator of forest maturity . In the Iberian Peninsula, records are limited to the northernmost zone .
Sampling collection: 10 (MACB 117673). GenBank accession number: OP479899.
Our specimens are: Pd–, K–, C–, KC+ (light yellow), UV–, containing zeorin and usnic acid. It is frequent and was located at different points of the area, developed on soil and rocks. The species has a wide distribution across Europe, Asia, North America and Macaronesia, although its exact distribution is not well-known . In the Iberian Peninsula, the majority of records are concentrated in the northwestern quadrant ; . have identified it as a polyphyletic taxon.
Sampling collections: 2 (MACB 117676), 8 (MACB 117672), 9 (MACB 117678), 10 (MACB 117674), 14 (MACB 117679). GenBank accession number: OP479900 for MACB 117678.
Our specimen is: Pd+ (reddish-orange), K–, C–, KC–, UV–, containing fumarprotocetraric acid. A single specimen was located growing on soil. The species is cosmopolitan , and is abundant in the Mediterranean Region. It has been widely cited in the Iberian Peninsula ; .
Sampling collection: 7 (MACB 117680).
The specimens are: Pd+ (yellow), K+ (yellow), C–, KC–, UV–, containing barbatic and thamnolic acids, which does not fit with what it is indicated by , although also consider this chemotype. Several specimens were found growing on moss debris on soil and bark of Quercus robur. This species has a subcosmopolitan distribution and is commonly found in oceanic areas . In the Iberian Peninsula, it is mostly recorded in the northwestern quadrant ; .
Sampling collections: 4 (MACB 117684), 7 (MACB 117683). Sequence code: OP479901 for MACB 117683.
Two chemotypes were found (fumarprotocetraric ± bourgeanic acids) both of them : Pd+ (red), K–, C–, KC–, UV–,. It is abundant in the study area. It was found on soil and occasionally among mosses, as well as on the bark of Salix atrocinerea. This is a subcosmopolitan species ; spread across all latitudes and also very common in much of the Mediterranean Region and the Iberian Peninsula ; .
Sampling collections: 1 (MACB 117687), 7 (MACB 117685), 8 (MACB 117686), 9 (MACB 117687, 117689), 14 (MACB 117690). GenBank accession number: OP479904 for MACB 117689.
Our specimens belong to chemotype: Pd–, K–, C–, KC–, UV+ (white), containing squamatic acid, and chemotype: Pd+ (yellow), K+ (yellow), KC±, (yellow), UV–, containing thamnolic and traces of barbatic acids. At once squamatic and thamnolic acids was found in some specimens. It is a very abundant species in some locations of the area of study. It grows on rocks, on soil, usually surrounded by mosses, although it was also found on Pinus radiata bark. According to , it is mainly distributed in the Northern Hemisphere, particularly in areas with an oceanic tendency . Previous studies have reported its presence in various locations in the western half of the Iberian Peninsula ; .
Sampling collections: 1 (MACB 117696, 117697), 2 (MACB 117693), 9 (MACB 117694, 117695), 10 (MACB 117691, 117692). GenBank accession numbers: OP479905 for MACB 117691, OP479906 for MACB 117693.
(Fig. 1d) Small squamules at the base of the podetia are frequently present. Our specimens belong to the chemotype: Pd–, K–, C–, KC–, UV+ (bluish-white), containing homosekikaic and sekikaic acids. It was found on soil, among mosses and on Pinus radiata bark in the highest altitude locality of the municipality (Alto de Campo do Couto). It is an uncommon species in the Northern Hemisphere and has also been observed in some parts of Australia . In the Iberian Peninsula, it has been found in scattered localities in the northern half . Our report represents the first record for Galicia.
Sampling collections: 1 (MACB 117791, 117792, 117793, 117794).
(Fig. 1e-f) The specimens belong to the chemotype: Pd+ (red), K+ (yellow), C–, KC–, UV–, with atranorin and fumarprotocetraric acid. Several specimens were found growing directly on soil and on various substrates (Alnus glutinosa, Castanea sativa, Pinus radiata, Quercus robur, and Salix atrocinerea), always among mosses. C. humilis has a subcosmopolitan distribution, but it is more widespread in temperate zones ; . There are numerous records of the species from the Iberian Peninsula ; .
Sampling collections: 1 (MACB 117704), 3 (MACB 117702), 4 (MACB 117784), 7 (MACB 117698), 8 (MACB 117700, 117701), 10 (MACB 117699), 12 (MACB 117781), 13 (MACB 117703). GenBank accession numbers: OP479907 for MACB 117698, OP479908 for MACB 117703.
Our specimens are: Pd+ (yellow), K+ (yellow-orange), C–, KC–, UV–, containing barbatic, thamnolic and didymic acids. In some specimens, only thamnolic acid has been detected. It is a very abundant species in the area. It grows on soil surrounded by mosses, on stumps and on bark of Pinus pinaster and P. radiata. The species has a wide distribution in both hemispheres, as reported by . It is found in hills and mountainous zones of the Eurosiberian Region, as well as wet areas of the Mediterranean Region . The majority of records from the Iberian Peninsula are concentrated in the north and northwestern quadrant ; .
Sampling collections: 1 (MACB 117737), 10 (MACB 117732), 11 (MACB 117735), 12 (MACB 117733), 13 (MACB 117734), 14 (MACB 117736).
Our specimen is: Pd–, K–, C–, KC+ (yellow), UV+ (light blue), containing usnic and perlatolic acids. It grows on soil and among mosses. The species is frequently found in humid and warm territories of the hills and montane levels of the Eurosiberian Region. In the Mediterranean basin, its distribution is restricted to pine forests, cork oak groves, and coastal shrub areas . Its population is currently declining due to the progressive alteration of its habitat. There are numerous records of this species from all over Portugal, the Balearic Islands, Catalonia, and some isolated localities in Galicia ; ; .
Sampling collections: 7 (MACB 117738). GenBank accession number: OP479910.
Our specimens are: Pd+ (orange-red), K–, C+ (reddish), KC+ (wine red), UV+ (pale blue), containing meroclorophaeic and fumarprotocetraric acids. It was found growing on soil and bases of Quercus robur, Q. suber and Pinus pinaster trunks. The taxon is subcosmopolitan, ranging from the Arctic to the temperate regions of the Northern Hemisphere, as well as scattered territories in Australasia and South America . It has also been reported in various locations in the Iberian Peninsula and other countries in the Mediterranean basin, although its presence is likely underestimated due to identification difficulties .
Sampling collections: 1 (MACB 117744, 117745), 4 (MACB 117742), 5 (MACB 117741), 7 (MACB 117743), 10 (MACB 117739, 117740), 14 (MACB 117746). GenBank accession numbers: OP479911 for MACB 117739.
(Fig. 1h-i This species is chemically variable. We have detected three chemotypes: 1) Pd–, K–, C–, KC± (pale yellow), UV+ (light blue), containing usnic and perlatolic acids; 2) Pd–, K–, C–, KC± (pale yellow), UV+ (light blue), containing only perlatolic acid; and 3) Pd+ (red), K–, C–, KC± (pale yellow), UV+ (light blue), containing fumarprotocetraric, perlatolic and usnic acids. The first chemotype is more prevalent in the northern half of the Iberian Peninsula, while the second chemotype is limited to a few locations in the western Iberian Peninsula (Galicia, Portugal, Salamanca), and Navarra . The third chemotype was previously considered a distinct species, Cladonia azorica Ahti, with a Macaronesian distribution. However, concluded that it belongs to C. portentosa. It is common in the study area, growing on soil accompanied by mosses.
The species has a subcosmopolitan distribution, although it is mainly found in the Northern Hemisphere . In Europe, it is more frequently found in the Eurosiberian Region, while in the Mediterranean Region, it is found at higher altitudes . There are numerous records of this species in northern Spain and Portugal ; . The third chemotype is the first record for the Iberian Peninsula and the second for the European continent, as it has also been found in the southwest of France .
Sampling collections: 1 (MACB 11755), 10 (MACB 117750, 117751, 117752, 117753), 4 (MACB 117754), 7 (MACB 117756), 8 (MACB 117757), 9 (MACB 117760), 15 (MACB 117759). GenBank accession numbers: OP479913 for MACB 117759, OP479914 for MACB 117750, OP479922 for MACB 117751, OP479923 for MACB 117757, OP479924 for MACB 117756.
Our specimens are: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. The character to differentiate C. pyxidata from other related taxa is the presence of large, unclustered, corticated podecia, which gradually open to form greenish-grey scyphi; these are covered with microscuamules and flat, peeled plates on the upper part and especially on the inside of the scyphi. Nevertheless, species is highly polymorphic and can be mistaken for both C. chlorophaea and some specimens of C. humilis. Molecular studies have revealed that it is a polyphyletic taxon , indicating the need for further revision. It was collected on soil and rocks growing in association with mosses. The species has a cosmopolitan distribution, as reported by , and has frequently cited in the Iberian Peninsula, according to and .
Sampling collections: 1 (MACB 117780, 117779), 2 (MACB 117782), 7 (MACB 117783), 10 (MACB 117785). GenBank accession numbers: OP479915 for MACB 117782, OP479925 for MACB 117783.
The specimens are: Pd + (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. This species is commonly found in the area and can be found on various substrates such as soil, mosses, rocky substrates, and trunks of Quercus suber and Pinus radiata. It has a cosmopolitan distribution, ranging from boreal regions to temperate zones , and is frequently found in the Mediterranean Region. There are numerous records of this species in the Iberian Peninsula ; .
Sampling collections: 1 (MACB 117773), 7 (MACB 117776, 117777), 8 (MACB 117775), 13 (MACB 117774). GenBank accession numbers: OP479916 for MACB 117776, OP479927 for MACB 117775.
The specimens are: Pd–, K+ (yellow), C–, KC–, UV–, containing atranorin and rangiformic acid. It is one of the most abundant species in the area. It grows on soil associated with mosses. C. rangiformis has a wide distribution in the Northern Hemisphere, ranging from the Arctic tundra to the Mediterranean Region and Macaronesia . There are numerous records of this species in the Iberian Peninsula ; .
Sampling collections: 1 (MACB 117770), 7 (MACB 117772, 117771). GenBank accession number: OP479917 for MACB 117772.
The specimens collected are: Pd–, K–, C–, KC–, UV+ (white), containing squamatic acid. Molecular phylogenetic studies suggest that it is a polyphyletic taxon . It is relatively abundant in some areas, growing directly on soil and surrounded by mosses, although it has also been found on Pinus radiata. Its distribution is subcosmopolitan , and it prefers areas with an oceanic or suboceanic tendency . The species has been reported in numerous localities throughout the Iberian Peninsula, although it appears to be more abundant in the Eurosiberian Region ; .
Sampling collections: 1 (MACB 117765, 117766, 117767), 7 (MACB 117764), 10 (MACB 117768, 117769).
Our specimens are: Pd+ (red), K+ (yellow), C–, KC–, UV–, containing fumarprotocetraric acid and atranorin. It grows on rocks usually accompanied by mosses. The species is distributed in western regions of Europe, although there is evidence of its presence in some localities near the Mediterranean coast. Most of the records are concentrated in the western third of the Iberian Peninsula, as reported by .
Sampling collections: 1 (MACB 117762), 2 (MACB 117763). GenBank accession number: OP479918 for MACB 117762.
The only specimen collected is: Pd+ (red), K–, C–, KC–, UV–, containing fumarprotocetraric acid. It was growing on soil among mosses. C. subulata has a subcosmopolitan distribution and is prevalent in continental areas of the Mediterranean Region. In the Iberian Peninsula, records are concentrated in the western half ; .
Sampling collection: 8 (MACB 117761). GenBank accession number: OP479919.
Our specimen is: Pd–, K–, C–, KC+ (yellow), medulla UV+ (white), containing usnic and squamatic acids. It is very rare in the area. Only one specimen was found growing on soil and surrounded by mosses. The distribution area of this species covers the European continent and isolated locations in Asia and the eastern coast of North America . In the Mediterranean Region, it is more common than subsp. uncialis . Records in the Iberian Peninsula are concentrated in the northwestern quadrant ; .
Sampling collection: 7 (MACB 117786). GenBank accession number: OP479921.
The following key is presented to identify the Cladonia species in O Rosal:
We thank the council of O Rosal, particularly the Department of the Environment, for granting us permission to conduct this study and for their prompt and courteous assistance throughout.
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